aurora scientific mini pid  (Aurora Scientific)

Journal: BMC Neuroscience

Article Title: Odor identity influences tracking of temporally patterned plumes in Drosophila

doi: 10.1186/1471-2202-12-62

Figure Lengend Snippet: Magnetically tethered flight assay and spatio-temporal measurement of odor plume tracking . (A) A hungry fly is tethered within a magnetic field that allows her to steer freely in the yaw plane. A wraparound LED display arena displays either a high contrast stationary grating pattern or moving features to "drag" the animal toward the odor nozzle. The nozzle delivers a smooth narrow odor plume which is drawn downward by an acrylic suction chamber. The fly's angular heading in the arena is tracked with IR video and software written in Matlab. (B) A miniature-photoionization detector (mini-PID) was used to make odor intensity measurements within a 9 mm 2 space occupied by the fly. Odor intensity, in arbitrary units, is plotted in pseudo-color. Each point in the grid was sampled 11 different times below the plane of a fly in flight and the resulting data was smoothed using piecewise linear interpolation in Matlab. (C)The plume was pulsed with a solenoid valve system, and the temporal fidelity of the pulse train was verified with mini-PID measurements made at the location of the tethered fly.

Article Snippet: To measure the temporal properties of odor stimuli, we used a miniature photoionization detector (mini-PID Aurora Scientific, Ontario, Canada).

Techniques: Software

Journal: BMC Neuroscience

Article Title: Odor identity influences tracking of temporally patterned plumes in Drosophila

doi: 10.1186/1471-2202-12-62

Figure Lengend Snippet: Odor delivery system faithfully reproduces the temporal stimuli . (A) A miniature photoionization detector was used to measure the temporal fidelity of a 2.5 Hz train of ethyl butyrate. Transients recover to baseline levels during, suggesting that the olfactometer faithfully produced temporally precise stimuli. (B) Flies were challenged to track a 10 -9 dilution of ethyl butyrate (a stimulus that registered a distinct signal recorded by PID measurements as shown in (C). At this dilution, the plume deviation was not significantly different than the response to water vapor alone. However, the responses to flies tracking undiluted ethyl butyrate were significantly different than flies receiving either a 10 -9 dilution of ethyl butyrate or water vapor. (t-test p < 0.01). n = 9 flies. (C) Distinct electrophysiological responses were observed to each pulse of ethyl butyrate which fully recovered to baseline when the solenoid valve controlling the water vapor was switched on. This refutes the possibility of any odor-induced contamination between ethyl butyrate pulses that might be below the PID detection limit. Shown are overlaid AFP traces recorded from 6 different preparations.

Article Snippet: To measure the temporal properties of odor stimuli, we used a miniature photoionization detector (mini-PID Aurora Scientific, Ontario, Canada).

Techniques: Produced